Measurement of Nattokinase Concentration in Blood

By Peter A. McCullough, MD, MPH

A doctor asked me at a meeting recently if Nattokinase can be measured in the blood, so I decided to investigate. Many of you know that Nattokinase is a key component of McCullough Protocol BSD (Base Spike Detoxification) to assist in the management of long-COVID and vaccine injury syndromes.

We know that Nattokinase is active after oral absorption because the D-dimer and other measures of hemostasis and thrombolysis change within an hour or two. However, a blood test to measure levels of the drug would be useful.

Tanikawa, et al, created a mouse model in order to develop the antibody pair that could be used in a enzyme-linked immunosorbent assay (ELISA). This is a standard method of measuring most proteins in whole blood, plasma, or sera.

Tanikawa T, Yu J, Hsu K, Chen S, Ishii A, Yokogawa T, Inoue Y, Kitamura M. Development of Novel Monoclonal Antibodies Against Nattokinase. Monoclon Antib Immunodiagn Immunother. 2023 Oct;42(5):153-156. doi: 10.1089/mab.2023.0012. Epub 2023 Oct 19. PMID: 37855913.

As you can see, the line of best fit indicating optical detection of immunofluorescence and antigen concentration of two different forms of Nattokinase was excellent.

The ELISA was developed using a combination of new mouse anti-nattokinase mAbs used as capture antibodies coated onto 96-well plates, with a peroxidase conjugated antibody used for detection. This ELISA enabled detection of nattokinase at 1 ng/mL.

This means the field of Nattokinase research will advance with pharmacokinetics and potentially target treatment studies to find the ideal dose and blood levels of Nattokinase needed to best manage the bourgeoning numbers of patients with post-acute sequalae after recurrent COVID-19 infection and or ill-advised COVID-19 vaccination.

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Peter A. McCullough, MD, MPH

President, McCullough Foundation

http://www.mcculloughfnd.org

Tanikawa T, Yu J, Hsu K, Chen S, Ishii A, Yokogawa T, Inoue Y, Kitamura M. Development of Novel Monoclonal Antibodies Against Nattokinase. Monoclon Antib Immunodiagn Immunother. 2023 Oct;42(5):153-156. doi: 10.1089/mab.2023.0012. Epub 2023 Oct 19. PMID: 37855913.

McCullough PA, Wynn C, Procter BC. Clinical Rationale for SARS-CoV-2 Base Spike Protein Detoxification in Post COVID-19 and Vaccine Injury Syndromes. Journal of American Physicians and Surgeons Volume 28 Number 3 Fall 2023, 90-93.